E2F5 Protein (E2F5) (His tag)
Quick Overview for E2F5 Protein (E2F5) (His tag) (ABIN2719897)
Target
See all E2F5 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This E2F5 protein is labelled with His tag.
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Characteristics
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- Recombinant human E2F5 (N-term HIS tag, transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
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E2F5 (E2F5) (AA 1-346) protein (His tag)
custom-made E2F5 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
E2F5 (E2F5) (AA 1-335) protein (His tag)custom-made E2F5 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
E2F5 (E2F5) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)E2F5 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the N-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- E2F5
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Alternative Name
- e2f5
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Background
- The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB. Alternative splicing results in multiple variants encoding different isoforms.
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Molecular Weight
- 37.4 kDa
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NCBI Accession
- NP_001942
Target
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