EEF1D Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2719944

This Recombinant EEF1D protein is produced in HEK-293 Cells. There is 1 publication available.

Quick Overview for EEF1D Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag) (ABIN2719944)

Target: EEF1D (Eukaryotic Translation Elongation Factor 1 delta (Guanine Nucleotide Exchange Protein) (EEF1D))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 2

Purification tag / Conjugate: This EEF1D protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human EEF1D (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Li, Wei, Rawle, Qin, Wang, Soares, Jin, Sivakumaran, Lin, Spann, Abbott, Harrich: "Specific Interaction between eEF1A and HIV RT Is Critical for HIV-1 Reverse Transcription and a Potential Anti-HIV Target." in: PLoS pathogens, Vol. 11, Issue 12, pp. e1005289, (2015) (PubMed).

Target Details

Target: EEF1D (Eukaryotic Translation Elongation Factor 1 delta (Guanine Nucleotide Exchange Protein) (EEF1D))

Alternative Name: Eef1d

Background: This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.[provided by RefSeq, Aug 2010].

Molecular Weight: 30.9 kDa

NCBI Accession: NP_001951