EIF4E Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2720093

Recombinant EIF4E protein expressed in HEK-293 Cells.

Quick Overview for EIF4E Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2720093)

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 1

Purification tag / Conjugate: This EIF4E protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human EIF4E (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Alternative Name: Eif4e

Background: The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants.

Molecular Weight: 24.9 kDa

NCBI Accession: NP_001959

Pathways: BCR Signaling