EPRS Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2720339

Recombinant EPRS protein expressed in HEK-293 Cells and has been mentioned in 1 publication.

Quick Overview for EPRS Protein (Myc-DYKDDDDK Tag) (ABIN2720339)

Target: EPRS (Glutamyl-Prolyl-tRNA Synthetase (EPRS))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This EPRS protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human EPRS protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Marquez-Jurado, Nogales, Zuñiga, Enjuanes, Almazán: "Identification of a gamma interferon-activated inhibitor of translation-like RNA motif at the 3' end of the transmissible gastroenteritis coronavirus genome modulating innate immune response." in: mBio, Vol. 6, Issue 2, pp. e00105, (2015) (PubMed).

Target Details

Target: EPRS (Glutamyl-Prolyl-tRNA Synthetase (EPRS))

Alternative Name: Eprs

Background: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. Alternative splicing has been observed for this gene, but the full-length nature and biological validity of the variant have not been determined.

Molecular Weight: 170.4 kDa

NCBI Accession: NP_004437