Sec8 Protein (His tag)

Catalog No. ABIN2720613

This Recombinant Sec8 protein is produced in Escherichia coli (E. coli).

Quick Overview for Sec8 Protein (His tag) (ABIN2720613)

Target: Sec8 (EXOC4) (Exocyst Complex Component 4 (EXOC4))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This Sec8 protein is labelled with His tag.

Characteristics:

• Recombinant human Exocyst complex component 4 (EXOC4), transcript variant 2, full length, with N-terminal HIS tag, expressed in E. coli, 50 μg (full length, N-term HIS tag, transcript variant 2) protein expressed in E.coli.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: Sec8 (EXOC4) (Exocyst Complex Component 4 (EXOC4))

Background: The protein encoded by this gene is a component of the exocyst complex, a multiple protein complex essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. The complex is also essential for the biogenesis of epithelial cell surface polarity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Molecular Weight: 53.7 kDa

NCBI Accession: NP_001032203

Pathways: Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis