EDDM3B Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2720839

This Recombinant EDDM3B protein is expressed in HEK-293 Cells.

Quick Overview for EDDM3B Protein (Myc-DYKDDDDK Tag) (ABIN2720839)

Target: EDDM3B (Epididymal Protein 3B (EDDM3B))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This EDDM3B protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human Family with sequence similarity 12, member B (epididymal) (FAM12B) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: EDDM3B (Epididymal Protein 3B (EDDM3B))

Alternative Name: Family with Sequence Similarity 12, Member B (Epididymal) (Fam12b)

Background: Testicular sperm are morphologically differentiated but are not progressively motile nor able to fertilize an egg. Post-testicular maturation requires exposure of spermatozoa to the microenvironment of the epididymal lumen. Spermatozoa undergo extensive changes in the epididymis, including enzymatic modifications, loss of pre-existing components and addition of new glycoproteins from epididymal secretions. These modifying proteins and enzymes are synthesized by epithelial cells lining the epididymal duct and secreted apically into the lumen, where they come into contact with, and may be absorbed onto, the sperm membranes. The proteins encoded by the genes in this cluster are synthesized and secreted by epididymal epithelial cells.

Molecular Weight: 17.4 kDa

NCBI Accession: NP_071755