FEN1 Protein (Myc-DYKDDDDK Tag)
Quick Overview for FEN1 Protein (Myc-DYKDDDDK Tag) (ABIN2721006)
Target
See all FEN1 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This FEN1 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human FEN1 / RAD2 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Flap Structure-Specific Endonuclease 1 (FEN1) (AA 1-378) protein (His tag)
custom-made FEN1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Flap Structure-Specific Endonuclease 1 (FEN1) (AA 1-380) protein (His tag)custom-made FEN1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Flap Structure-Specific Endonuclease 1 (FEN1) (AA 1-380) protein (GST tag)FEN1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))
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Alternative Name
- Fen1,rad2
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Background
- The protein encoded by this gene removes 5&apos overhanging flaps in DNA repair and processes the 5&apos ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5&apos end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
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Molecular Weight
- 42.4 kDa
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NCBI Accession
- NP_004102
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Pathways
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA
Target
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