FGF12 Protein (Transcript Variant 2) (His tag)
Quick Overview for FGF12 Protein (Transcript Variant 2) (His tag) (ABIN2721057)
Target
See all FGF12 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 2
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Purification tag / Conjugate
- This FGF12 protein is labelled with His tag.
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Characteristics
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- Recombinant human FGF12 (transcript variant 2) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
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Endotoxin Level
- < 0.1 EU per μg protein as determined by LAL test
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Fibroblast Growth Factor 12 (FGF12) (AA 1-243) protein (His tag)
custom-made FGF12 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Fibroblast Growth Factor 12 (FGF12) (AA 1-243) protein (His tag)custom-made FGF12 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Fibroblast Growth Factor 12 (FGF12) proteinFGF12 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by reducing SDS-PAGE. AM, ADA, IA, ScA
Fibroblast Growth Factor 12 (FGF12) (AA 1-181) protein (GST tag)FGF12 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM Phosphate buffer, 150 mM NaCl, 5 mM EDTA, pH 7.5. Stable for at least 6 months from date of receipt under proper storage and handling conditions.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- FGF12 (Fibroblast Growth Factor 12 (FGF12))
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Alternative Name
- Fgf12
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Background
- The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This growth factor lacks the N-terminal signal sequence present in most of the FGF family members, but it contains clusters of basic residues that have been demonstrated to act as a nuclear localization signal. When transfected into mammalian cells, this protein accumulated in the nucleus, but was not secreted. The specific function of this gene has not yet been determined. Two alternatively spliced transcript variants encoding distinct isoforms have been reported.
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Molecular Weight
- 20.5 kDa
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NCBI Accession
- NP_004104
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Pathways
- Negative Regulation of Transporter Activity
Target
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