FUBP1 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2721443

Recombinant FUBP1 protein expressed in HEK-293 Cells and has been mentioned in 1 publication.

Quick Overview for FUBP1 Protein (Myc-DYKDDDDK Tag) (ABIN2721443)

Target: FUBP1 (Far Upstream Element (FUSE) Binding Protein 1 (FUBP1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This FUBP1 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human FUBP1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Miro, Laaref, Rofidal, Lagrafeuille, Hem, Thorel, Méchin, Mamchaoui, Mouly, Claustres, Tuffery-Giraud: "FUBP1: a new protagonist in splicing regulation of the DMD gene." in: Nucleic acids research, Vol. 43, Issue 4, pp. 2378-89, (2015) (PubMed).

Target Details

Target: FUBP1 (Far Upstream Element (FUSE) Binding Protein 1 (FUBP1))

Alternative Name: Fubp1

Background: The protein encoded by this gene is a single stranded DNA-binding protein that binds to multiple DNA elements, including the far upstream element (FUSE) located upstream of c-myc. Binding to FUSE occurs on the non-coding strand, and is important to the regulation of c-myc in undifferentiated cells. This protein contains three domains, an amphipathic helix N-terminal domain, a DNA-binding central domain, and a C-terminal transactivation domain that contains three tyrosine-rich motifs. The N-terminal domain is thought to repress the activity of the C-terminal domain. This protein is also thought to bind RNA, and contains 3'-5' helicase activity with in vitro activity on both DNA-DNA and RNA-RNA duplexes. Aberrant expression of this gene has been found in malignant tissues, and this gene is important to neural system and lung development. Binding of this protein to viral RNA is thought to play a role in several viral diseases, including hepatitis C and hand, foot and mouth disease. Alternative splicing results in multiple transcript variants.

Molecular Weight: 67.4 kDa

NCBI Accession: NP_003893