G-CSF Protein (Transcript Variant 3)

Catalog No. ABIN2721516

This Recombinant G-CSF protein is produced in HEK-293 Cells.

Quick Overview for G-CSF Protein (Transcript Variant 3) (ABIN2721516)

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)

Purity: > 95 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: Transcript Variant 3

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human G-CSF (transcript variant 3) protein expressed in HEK293.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Endotoxin Level: Less than 0.01 ng per μg protein as determined by the LAL method.

Biological Activity Comment: The ED50 is 0.02 - 0.08 ng/ml, corresponding to a specific activity of 1.25 - 5.0 x 10^7 units/mg, determined by M-NFS60 cell proliferation induced by human G-CSF in a dose dependent manner.

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Handling

Concentration: > 50 μg/mL

Buffer: 1 x PBS

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Alternative Name: G-Csf

Background: The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene.

Molecular Weight: 18.6 kDa

NCBI Accession: NP_757374

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization