GPT2 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
(1 reference)Quick Overview for GPT2 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2722205)
Target
See all GPT2 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Purification tag / Conjugate
- This GPT2 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human GPT2 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Glutamic Pyruvate Transaminase (Alanine Aminotransferase) 2 (GPT2) (AA 1-522) protein (His tag)
custom-made GPT2 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Glutamic Pyruvate Transaminase (Alanine Aminotransferase) 2 (GPT2) (AA 1-523) protein (GST tag)GPT2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Glutamic Pyruvate Transaminase (Alanine Aminotransferase) 2 (GPT2) protein (His tag)GPT2 Origin: Rat Host: Baculovirus infected Insect Cells Recombinant > 95 % as determined by SDS-PAGE
Glutamic Pyruvate Transaminase (Alanine Aminotransferase) 2 (GPT2) proteinGPT2 Origin: Human Host: Escherichia coli (E. coli) Recombinant SDS
Glutamic Pyruvate Transaminase (Alanine Aminotransferase) 2 (GPT2) (AA 1-523) protein (His-IF2DI Tag)GPT2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Glutamic Pyruvate Transaminase (Alanine Aminotransferase) 2 (GPT2) (AA 1-523) protein (His tag)GPT2 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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: "Quantitative liver-specific protein fingerprint in blood: a signature for hepatotoxicity." in: Theranostics, Vol. 4, Issue 2, pp. 215-28, (2014) (PubMed).
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- GPT2 (Glutamic Pyruvate Transaminase (Alanine Aminotransferase) 2 (GPT2))
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Alternative Name
- Gpt2
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Background
- This gene encodes a mitochondrial alanine transaminase, a pyridoxal enzyme that catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate. Alanine transaminases play roles in gluconeogenesis and amino acid metabolism in many tissues including skeletal muscle, kidney, and liver. Activating transcription factor 4 upregulates this gene under metabolic stress conditions in hepatocyte cell lines. A loss of function mutation in this gene has been associated with developmental encephalopathy. Alternative splicing results in multiple transcript variants.
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Molecular Weight
- 57.7 kDa
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NCBI Accession
- NP_597700
Target
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