HDAC4 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2722509

This Recombinant HDAC4 protein is produced in HEK-293 Cells. There is 1 publication available.

Quick Overview for HDAC4 Protein (Myc-DYKDDDDK Tag) (ABIN2722509)

Target: HDAC4 (Histone Deacetylase 4 (HDAC4))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This HDAC4 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human HDAC4 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Yang, Li, Chen, Yuan, Dong, Zhang, Wu, Wang: "PARP-1 mediates LPS-induced HMGB1 release by macrophages through regulation of HMGB1 acetylation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 193, Issue 12, pp. 6114-23, (2014) (PubMed).

Target Details

Target: HDAC4 (Histone Deacetylase 4 (HDAC4))

Alternative Name: Hdac4

Background: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3.

Molecular Weight: 118.9 kDa

NCBI Accession: NP_006028

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process