Histone Cluster 3, H3 (HIST3H3) Protein

Catalog No. ABIN2722818

This Recombinant protein is produced in Escherichia coli (E. coli).

Quick Overview for Histone Cluster 3, H3 (HIST3H3) Protein (ABIN2722818)

Target: Histone Cluster 3, H3 (HIST3H3)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Antibody Production (AbP), Standard (STD)

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Characteristics:

• Recombinant human Histone H3.1t protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Endotoxin Level: <0.1 EU per 1 μg of the protein by the LAL

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Restrictions: For Research Use only

Handling

Buffer: Lyophilized from a sterile solution containing 20 mM phosphate buffer, pH 7.4. Stable for at least 6 months from date of receipt under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: Histone Cluster 3, H3 (HIST3H3)

Alternative Name: Histone h3.1t

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Molecular Weight: 15.5 kDa

NCBI Accession: NP_003484