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Histone H4 Protein (Myc-DYKDDDDK Tag)

This Recombinant Histone H4 protein is produced in HEK-293 Cells.
Catalog No. ABIN2722824

Quick Overview for Histone H4 Protein (Myc-DYKDDDDK Tag) (ABIN2722824)

Target

See all Histone H4 Proteins
Histone H4

Protein Type

Recombinant

Origin

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Human

Source

  • 38
  • 11
HEK-293 Cells

Application

Antibody Production (AbP), Standard (STD)

Purity

> 80 % as determined by SDS-PAGE and Coomassie blue staining
  • Purification tag / Conjugate

    This Histone H4 protein is labelled with Myc-DYKDDDDK Tag.

    Characteristics

    • Recombinant human Histone H4 protein expressed in HEK293 cells.
    • Produced with end-sequenced ORF clone
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  • Application Notes

    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays

    Comment

    The tag is located at the C-terminal.

    Restrictions

    For Research Use only
  • Concentration

    50 μg/mL

    Buffer

    25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

    Storage

    -80 °C

    Storage Comment

    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • Target

    Histone H4

    Background

    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails instead, they contain a palindromic termination element.

    Molecular Weight

    11.2 kDa

    NCBI Accession

    NP_778224
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