HNF4A Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)
Quick Overview for HNF4A Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag) (ABIN2722902)
Target
See all HNF4A ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 2
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Purification tag / Conjugate
- This HNF4A protein is labelled with Myc-DYKDDDDK Tag.
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Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
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Characteristics
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- Recombinant human HNF4 alpha / TCF14 (transcript variant 2) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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Biological Activity Comment
- HNF4A Activity Verified in a DNA-binding Assay: HNF4A (, transcript variant 2) activity was measured in a colorimetric DNA-binding assay. Purified HNF4A protein containing a C-terminal MYC/DDK tag was incubated with biotinylated double-stranded oligonucleotide containing the HNF4A consensus DNA-binding sequence (see below). Following incubation, the reaction was transferred to a streptavidin-coated microplate to allow capture of the DNA-protein complex. After washing, the captured protein was detected with an anti-DDK peroxidase conjugate and colorimetric signal detection with TMB. Specificity of the protein-DNA interaction was confirmed by carrying out the binding in the presence of an unlabeled competitor oligonucleotide and by comparison to binding to an oligonucleotide containing a mutation in the consensus binding sequence.
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- > 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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Hepatocyte nuclear factor (HNF) 4α transactivation of cytochrome P450 (Cyp) 2d40 promoter is enhanced during pregnancy in mice." in: Biochemical pharmacology, Vol. 94, Issue 1, pp. 46-52, (2015) (PubMed).
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Hepatocyte nuclear factor (HNF) 4α transactivation of cytochrome P450 (Cyp) 2d40 promoter is enhanced during pregnancy in mice." in: Biochemical pharmacology, Vol. 94, Issue 1, pp. 46-52, (2015) (PubMed).
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- HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))
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Alternative Name
- Hnf4 Alpha,tcf14
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Background
- Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine. [UniProtKB/Swiss-Prot Function]
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Molecular Weight
- 52.6 kDa
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NCBI Accession
- NP_000448
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Pathways
- AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process
Target
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