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HNF4A Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

This Recombinant HNF4A protein is produced in HEK-293 Cells. There is 1 publication available.
Catalog No. ABIN2722902

Quick Overview for HNF4A Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag) (ABIN2722902)

Target

See all HNF4A Proteins
HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Protein Type

Recombinant

Biological Activity

Active

Origin

  • 10
  • 2
  • 1
  • 1
Human

Source

  • 6
  • 2
  • 2
  • 2
  • 2
HEK-293 Cells

Application

Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)

Purity

> 80 % as determined by SDS-PAGE and Coomassie blue staining
  • Protein Characteristics

    Transcript Variant 2

    Purification tag / Conjugate

    This HNF4A protein is labelled with Myc-DYKDDDDK Tag.

    Specificity

    Optimal preservation of protein structure, post-translational modifications and functions.

    Characteristics

    • Recombinant human HNF4 alpha / TCF14 (transcript variant 2) protein expressed in HEK293 cells.
    • Produced with end-sequenced ORF clone
    • Tested for bioactivity.

    Biological Activity Comment

    HNF4A Activity Verified in a DNA-binding Assay: HNF4A (, transcript variant 2) activity was measured in a colorimetric DNA-binding assay. Purified HNF4A protein containing a C-terminal MYC/DDK tag was incubated with biotinylated double-stranded oligonucleotide containing the HNF4A consensus DNA-binding sequence (see below). Following incubation, the reaction was transferred to a streptavidin-coated microplate to allow capture of the DNA-protein complex. After washing, the captured protein was detected with an anti-DDK peroxidase conjugate and colorimetric signal detection with TMB. Specificity of the protein-DNA interaction was confirmed by carrying out the binding in the presence of an unlabeled competitor oligonucleotide and by comparison to binding to an oligonucleotide containing a mutation in the consensus binding sequence.
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    Product
    Expression System
    Conjugate
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    Expression System HEK-293 Cells
    Conjugate His tag
    Origin Human
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    Conjugate Strep Tag
    Origin Human
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  • Application Notes

    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays
    Protein-protein interaction
    In vitro biochemical assays and cell-based functional assays

    Comment

    The tag is located at the C-terminal.

    Restrictions

    For Research Use only
  • Concentration

    > 50 μg/mL

    Buffer

    25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

    Storage

    -80 °C

    Storage Comment

    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • Ning, Koh, Pan, Jeong: "Hepatocyte nuclear factor (HNF) 4α transactivation of cytochrome P450 (Cyp) 2d40 promoter is enhanced during pregnancy in mice." in: Biochemical pharmacology, Vol. 94, Issue 1, pp. 46-52, (2015) (PubMed).

  • Target

    HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

    Alternative Name

    Hnf4 Alpha,tcf14

    Background

    Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine. [UniProtKB/Swiss-Prot Function]

    Molecular Weight

    52.6 kDa

    NCBI Accession

    NP_000448

    Pathways

    AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process
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