ID3 Protein (Myc-DYKDDDDK Tag)
(1 reference)Quick Overview for ID3 Protein (Myc-DYKDDDDK Tag) (ABIN2723242)
Target
See all ID3 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This ID3 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human ID3 / BHLHB25 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Inhibitor of DNA Binding 3, Dominant Negative Helix-Loop-Helix Protein (ID3) (AA 1-119) protein (His tag)
custom-made ID3 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Inhibitor of DNA Binding 3, Dominant Negative Helix-Loop-Helix Protein (ID3) (AA 1-119) protein (His tag)custom-made ID3 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Inhibitor of DNA Binding 3, Dominant Negative Helix-Loop-Helix Protein (ID3) (AA 1-119) protein (GST tag)ID3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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: "PCB153-induced overexpression of ID3 contributes to the development of microvascular lesions." in: PLoS ONE, Vol. 9, Issue 8, pp. e104159, (2014) (PubMed).
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: "PCB153-induced overexpression of ID3 contributes to the development of microvascular lesions." in: PLoS ONE, Vol. 9, Issue 8, pp. e104159, (2014) (PubMed).
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- ID3 (Inhibitor of DNA Binding 3, Dominant Negative Helix-Loop-Helix Protein (ID3))
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Alternative Name
- Id3,bhlhb25
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Background
- The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with other HLH proteins. However, the encoded protein lacks a basic DNA-binding domain and therefore inhibits the DNA binding of any HLH protein with which it interacts.
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Molecular Weight
- 12.8 kDa
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NCBI Accession
- NP_002158
Target
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