IDE Protein (Myc-DYKDDDDK Tag)
(1 reference)Quick Overview for IDE Protein (Myc-DYKDDDDK Tag) (ABIN2723516)
Target
See all IDE ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This IDE protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human Insulin-degrading enzyme protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Insulin-Degrading Enzyme (IDE) (AA 1-1019) protein (His tag)
custom-made IDE Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Insulin-Degrading Enzyme (IDE) (AA 1-1019) protein (His tag)custom-made IDE Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Insulin-Degrading Enzyme (IDE) (AA 920-1019) protein (GST tag)IDE Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Insulin-Degrading Enzyme (IDE) (AA 1-1019) protein (GST tag)IDE Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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: "Analysis of the proteolysis of bioactive peptides using a peptidomics approach." in: Nature protocols, Vol. 8, Issue 9, pp. 1730-42, (2013) (PubMed).
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: "Analysis of the proteolysis of bioactive peptides using a peptidomics approach." in: Nature protocols, Vol. 8, Issue 9, pp. 1730-42, (2013) (PubMed).
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- IDE (Insulin-Degrading Enzyme (IDE))
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Alternative Name
- Insulin-Degrading Enzyme
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Background
- This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.[provided by RefSeq, Sep 2009].
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Molecular Weight
- 117.8 kDa
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NCBI Accession
- NP_004960
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Pathways
- SARS-CoV-2 Protein Interactome
Target
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