IGF2 Protein (Transcript Variant 2)
Quick Overview for IGF2 Protein (Transcript Variant 2) (ABIN2723527)
Target
See all IGF2 ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 2
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Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
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Characteristics
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- Recombinant human Insulin-like growth factor II / IGF2 (transcript variant 2) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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Endotoxin Level
- Less than 0.01 ng per μg protein as determined by the LAL method.
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Biological Activity Comment
- The ED50 is 10 - 30 ng/ml, corresponding to a specific activity of 0.3 - 1 x 10^5 units/mg, determined by the dose dependent stimulation of MCF-7 cell proliferation.
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
Restrictions
- For Research Use only
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Concentration
- > 50 μg/mL
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Buffer
- 1 x PBS
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- IGF2 (Insulin-Like Growth Factor 2 (IGF2))
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Alternative Name
- Insulin-Like Growth Factor Ii,igf2
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Molecular Weight
- 7.4 kDa
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NCBI Accession
- NP_001116208
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Pathways
- Hormone Activity, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Activated T Cell Proliferation
Target
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