IL27 Protein

Catalog No. ABIN2723689

This Recombinant IL27 protein is expressed in HEK-293 Cells.

Quick Overview for IL27 Protein (ABIN2723689)

Target: IL27 (Interleukin-27 Subunit alpha (IL27))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Application: Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)

Purity: Purity is > 95 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human Interleukin-27 / IL27A protein expressed in HEK293.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Endotoxin Level: Less than 0.01 ng per μg protein as determined by the LAL method.

Biological Activity Comment: The ED50 is 5 - 10 ng/ml, corresponding to a specific activity of 1.0 - 2.0 x 10^5 units/mg, determined by inhibition of IL-2 production induced by mouse IL-27 in mouse splenocytes activated with anti-CD3 and anti-CD28 antibodies.

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Handling

Concentration: > 50 μg/mL

Buffer: 20 mM NaH2PO4, pH 6, 0.60 M NaCl

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: IL27 (Interleukin-27 Subunit alpha (IL27))

Alternative Name: Interleukin-27,il27a

Molecular Weight: 49 kDa

NCBI Accession: NP_663611