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Keratin 77 Protein (KRT77) (His tag)

Recombinant Keratin 77 protein expressed in Escherichia coli (E. coli).
Catalog No. ABIN2724131

Quick Overview for Keratin 77 Protein (KRT77) (His tag) (ABIN2724131)

Target

See all Keratin 77 (KRT77) Proteins
Keratin 77 (KRT77)

Protein Type

Recombinant

Origin

  • 4
  • 2
  • 1
Human

Source

  • 2
  • 2
  • 2
  • 1
Escherichia coli (E. coli)

Application

Antibody Production (AbP), Standard (STD)

Purity

> 80 % as determined by SDS-PAGE and Coomassie blue staining
  • Purification tag / Conjugate

    This Keratin 77 protein is labelled with His tag.

    Characteristics

    • Recombinant human Cytokeratin-1B / KRT77 (full length, N-term HIS tag) protein expressed in E.coli.
    • Produced with end-sequenced ORF clone
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  • Application Notes

    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays

    Comment

    The tag is located at the N-terminal.

    Restrictions

    For Research Use only
  • Concentration

    50 μg/mL

    Buffer

    25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.

    Storage

    -80 °C

    Storage Comment

    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • Target

    Keratin 77 (KRT77)

    Background

    Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. This gene encodes an epithelial keratin that is expressed in the skin and eccrine sweat glands. The type II keratins are clustered in a region of chromosome 12q13.[provided by RefSeq, Jun 2009].

    Molecular Weight

    61.7 kDa

    NCBI Accession

    NP_778253
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