KLHL3 Protein (Myc-DYKDDDDK Tag)
Quick Overview for KLHL3 Protein (Myc-DYKDDDDK Tag) (ABIN2724309)
Target
See all KLHL3 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This KLHL3 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human KLHL3 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- KLHL3 (Kelch-Like 3 (KLHL3))
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Alternative Name
- Klhl3
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Background
- This gene is ubiquitously expressed and encodes a full-length protein which has an N-terminal BTB domain followed by a BACK domain and six kelch-like repeats in the C-terminus. These kelch-like repeats promote substrate ubiquitination of bound proteins via interaction of the BTB domain with the CUL3 (cullin 3) component of a cullin-RING E3 ubiquitin ligase (CRL) complex. Muatations in this gene cause pseudohypoaldosteronism type IID (PHA2D) a rare Mendelian syndrome featuring hypertension, hyperkalaemia and metabolic acidosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
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Molecular Weight
- 64.8 kDa
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NCBI Accession
- NP_059111
Target
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