LIM Domain Binding 3 Protein Protein (Transcript Variant 3) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2724563

Recombinant LIM Domain Binding 3 Protein protein expressed in HEK-293 Cells.

Quick Overview for LIM Domain Binding 3 Protein Protein (Transcript Variant 3) (Myc-DYKDDDDK Tag) (ABIN2724563)

Target: LIM Domain Binding 3 Protein (LDB3) (LIM Domain Binding 3 (LDB3))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 3

Purification tag / Conjugate: This LIM Domain Binding 3 Protein protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human LDB3 (transcript variant 3) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: LIM Domain Binding 3 Protein (LDB3) (LIM Domain Binding 3 (LDB3))

Alternative Name: Ldb3

Background: This gene encodes a PDZ domain-containing protein. PDZ motifs are modular protein-protein interaction domains consisting of 80-120 amino acid residues. PDZ domain-containing proteins interact with each other in cytoskeletal assembly or with other proteins involved in targeting and clustering of membrane proteins. The protein encoded by this gene interacts with alpha-actinin-2 through its N-terminal PDZ domain and with protein kinase C via its C-terminal LIM domains. The LIM domain is a cysteine-rich motif defined by 50-60 amino acids containing two zinc-binding modules. This protein also interacts with all three members of the myozenin family. Mutations in this gene have been associated with myofibrillar myopathy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been identified all isoforms have N-terminal PDZ domains while only longer isoforms (1, 2 and 5) have C-terminal LIM domains.

Molecular Weight: 35.5 kDa

NCBI Accession: NP_001073584