LIPE Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2724771

This Recombinant LIPE protein is produced in HEK-293 Cells.

Quick Overview for LIPE Protein (Myc-DYKDDDDK Tag) (ABIN2724771)

Target: LIPE (Lipase, Hormone-Sensitive (LIPE))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This LIPE protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human LIPE protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: LIPE (Lipase, Hormone-Sensitive (LIPE))

Alternative Name: Lipe

Background: The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids.

Molecular Weight: 116.4 kDa

NCBI Accession: NP_005348

Pathways: AMPK Signaling, Monocarboxylic Acid Catabolic Process, Lipid Metabolism