MIA Protein (Transcript Variant 1)
Quick Overview for MIA Protein (Transcript Variant 1) (ABIN2725964)
Target
See all MIA ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
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Characteristics
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- Recombinant human MIA (transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
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Biological Activity Comment
- ED50 was determined by the dose-dependant proliferation of the human A375 melanoma cell line. The expected ED50 for this effect is 4-6ug/mL.
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Melanoma Inhibitory Activity (MIA) (Active) protein
MIA Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Melanoma Inhibitory Activity (MIA) (AA 25-131) protein (His-IF2DI Tag)MIA Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Melanoma Inhibitory Activity (MIA) (AA 1-131) protein (GST tag)MIA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Melanoma Inhibitory Activity (MIA) protein (Myc-DYKDDDDK Tag)MIA Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
Restrictions
- For Research Use only
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Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
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Handling Advice
- Resuspend the protein in the desired concentration in proper buffer
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- MIA (Melanoma Inhibitory Activity (MIA))
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Alternative Name
- Mia
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Background
- Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas. [UniProtKB/Swiss-Prot Function]
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Molecular Weight
- 12.2 kDa
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NCBI Accession
- NP_006524
Target
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