MRPL53 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2726070

This Recombinant MRPL53 protein is produced in HEK-293 Cells.

Quick Overview for MRPL53 Protein (Myc-DYKDDDDK Tag) (ABIN2726070)

Target: MRPL53 (Mitochondrial Ribosomal Protein L53 (MRPL53))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This MRPL53 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human Mitochondrial ribosomal protein L53 (MRPL53), nuclear gene encoding mitochondrial protein protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: MRPL53 (Mitochondrial Ribosomal Protein L53 (MRPL53))

Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 1p.

Molecular Weight: 11.9 kDa

NCBI Accession: NP_444278