MYL12B Protein (Transcript Variant 4) (Myc-DYKDDDDK Tag)
Quick Overview for MYL12B Protein (Transcript Variant 4) (Myc-DYKDDDDK Tag) (ABIN2726714)
Target
See all MYL12B ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 4
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Purification tag / Conjugate
- This MYL12B protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human Myosin, light chain 12B, regulatory (MYL12B), transcript variant 4 (transcript variant 4) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Myosin, Light Chain 12B, Regulatory (MYL12B) (AA 1-172) protein (His tag)
custom-made MYL12B Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Myosin, Light Chain 12B, Regulatory (MYL12B) (AA 1-172) protein (His tag)custom-made MYL12B Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Myosin, Light Chain 12B, Regulatory (MYL12B) (AA 1-172) protein (GST tag)MYL12B Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Myosin, Light Chain 12B, Regulatory (MYL12B) (AA 1-172) protein (His-IF2DI Tag)MYL12B Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Myosin, Light Chain 12B, Regulatory (MYL12B) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)MYL12B Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- MYL12B (Myosin, Light Chain 12B, Regulatory (MYL12B))
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Background
- The activity of nonmuscle myosin II (see MYH9 MIM 160775) is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments (Iwasaki et al., 2001 [PubMed 11942626]).[supplied by OMIM, Mar 2008].
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Molecular Weight
- 17.6 kDa
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NCBI Accession
- NP_001138418
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Pathways
- Feeding Behaviour
Target
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