NAGPA Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2726733

This Recombinant NAGPA protein is produced in HEK-293 Cells.

Quick Overview for NAGPA Protein (Myc-DYKDDDDK Tag) (ABIN2726733)

Target: NAGPA (N-Acetylglucosamine-1-phosphodiester alpha-N-Acetylglucosaminidase (NAGPA))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This NAGPA protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human NAGPA protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: NAGPA (N-Acetylglucosamine-1-phosphodiester alpha-N-Acetylglucosaminidase (NAGPA))

Background: Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. This gene encodes the enzyme that catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal hydrolases. Commonly known as 'uncovering enzyme' or UCE, this enzyme removes N-acetyl-D-glucosamine (GlcNAc) residues from GlcNAc-alpha-P-mannose moieties and thereby produces the recognition marker. The encoded preproprotein is proteolytically processed by furin to generate the mature enzyme, a homotetramer of two disulfide-linked homodimers. Mutations in this gene are associated with developmental stuttering in human patients.

Molecular Weight: 53.3 kDa

NCBI Accession: NP_057340