Quick Overview for ME1 Protein (Myc-DYKDDDDK Tag) (ABIN2726777)
Target
See all ME1 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This ME1 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human NADP-ME protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1) (AA 1-572) protein (His tag)
custom-made ME1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1) (AA 1-572) protein (His tag)custom-made ME1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1) (AA 1-572) protein (GST tag)ME1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1) (AA 226-572) protein (His tag)ME1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- ME1 (Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1))
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Alternative Name
- Nadp-Me
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Background
- This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.
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Molecular Weight
- 64 kDa
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NCBI Accession
- NP_002386
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Pathways
- Regulation of Lipid Metabolism by PPARalpha
Target
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