Nanog Protein (TAT tag)
Quick Overview for Nanog Protein (TAT tag) (ABIN2726789)
Target
See all Nanog (NANOG) ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This Nanog protein is labelled with TAT tag.
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Characteristics
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- Recombinant human NANOG protein expressed in E. coli.
- Produced with end-sequenced ORF clone
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Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
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Nanog Homeobox (NANOG) (AA 1-305) protein (His tag)
custom-made NANOG Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Nanog Homeobox (NANOG) (AA 1-305) protein (His tag)custom-made NANOG Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Nanog Homeobox (NANOG) (AA 2-305) protein (His-IF2DI Tag)NANOG Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Nanog Homeobox (NANOG) (AA 1-305) proteinNANOG Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % pure SDS
Nanog Homeobox (NANOG) (AA 1-305) proteinNANOG Origin: Human Host: Escherichia coli (E. coli) > 90 % by SDS - PAGE SDS
Nanog Homeobox (NANOG) (AA 1-154) protein (His tag)NANOG Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90% by SDS-PAGE SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Restrictions
- For Research Use only
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Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
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Handling Advice
- Resuspend the protein in the desired concentration in proper buffer
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Nanog (NANOG) (Nanog Homeobox (NANOG))
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Alternative Name
- Nanog
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Background
- The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene.
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Molecular Weight
- 36.2 kDa
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NCBI Accession
- NP_079141
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Pathways
- Stem Cell Maintenance
Target
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