HECW2 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2727068

Recombinant HECW2 protein expressed in HEK-293 Cells.

Quick Overview for HECW2 Protein (Myc-DYKDDDDK Tag) (ABIN2727068)

Target: HECW2 (HECT, C2 and WW Domain Containing E3 Ubiquitin Protein Ligase 2 (HECW2))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This HECW2 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human NEDL2 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: HECW2 (HECT, C2 and WW Domain Containing E3 Ubiquitin Protein Ligase 2 (HECW2))

Alternative Name: Nedl2

Background: This gene encodes a member of a family of E3 ubiquitin ligases which plays an important role in the proliferation, migration and differentiation of neural crest cells as a regulator of glial cell line-derived neurotrophic factor (GDNF)/Ret signaling. This gene also plays an important role in angiogenesis through stabilization of endothelial cell-to-cell junctions as a regulator of angiomotin-like 1 stability. The encoded protein contains an N-terminal calcium/lipid-binding (C2) domain involved in membrane targeting, two-four WW domains responsible for cellular localization and substrate recognition, and a C-terminal homologous with E6-associated protein C-terminus (HECT) catalytic domain. Naturally occurring mutations in this gene are associated with neurodevelopmental delay, hypotonia, and epilepsy. The decreased expression of this gene in the aganglionic colon is associated with Hirschsprung&aposs disease. Alternative splicing results in multiple transcript variants.

Molecular Weight: 175.6 kDa

NCBI Accession: NP_065811