Nerve Growth Factor Protein (NGF)

Catalog No. ABIN2727244

This Recombinant Nerve Growth Factor protein is expressed in Escherichia coli (E. coli).

Quick Overview for Nerve Growth Factor Protein (NGF) (ABIN2727244)

Target: Nerve Growth Factor (NGF)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human NGF protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Endotoxin Level: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: determined by its ability to stimulate chick E9 DRG neurite outgrowth. ED50 is less than or equal to 1.0 ng/ml, corresponding to a specific activity of > 1 x 10^6 units/mg.

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Handling

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

Handling Advice: Resuspend the protein in the desired concentration in proper buffer

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: Nerve Growth Factor (NGF)

Alternative Name: Ngf

Background: This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene&aposs expression is associated with allergic rhinitis.

Molecular Weight: 13.5 kDa

NCBI Accession: NP_002497

Pathways: Regulation of Cell Size