NONO Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)
Quick Overview for NONO Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag) (ABIN2727463)
Target
See all NONO ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 2
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Purification tag / Conjugate
- This NONO protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human NONO / NMT55 (transcript variant 2) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Non-POU Domain Containing, Octamer-Binding (NONO) (AA 1-473) protein (His tag)
custom-made NONO Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Non-POU Domain Containing, Octamer-Binding (NONO) (AA 1-471) protein (His tag)custom-made NONO Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Non-POU Domain Containing, Octamer-Binding (NONO) (AA 101-210) protein (GST tag)NONO Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Non-POU Domain Containing, Octamer-Binding (NONO) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)NONO Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Non-POU Domain Containing, Octamer-Binding (NONO) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)NONO Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- NONO (Non-POU Domain Containing, Octamer-Binding (NONO))
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Alternative Name
- Nono,nmt55
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Background
- This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16.
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Molecular Weight
- 54.1 kDa
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NCBI Accession
- NP_031389
Target
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