Nucleolin Protein (NCL) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2727708

This Recombinant Nucleolin protein is produced in HEK-293 Cells. There are 3 publications available.

Quick Overview for Nucleolin Protein (NCL) (Myc-DYKDDDDK Tag) (ABIN2727708)

Target: Nucleolin (NCL)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This Nucleolin protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human Nucleolin protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Hernández, Sandoval-Jaime, Sosnovtsev, Green, Gutiérrez-Escolano: "Nucleolin promotes in vitro translation of feline calicivirus genomic RNA." in: Virology, Vol. 489, pp. 51-62, (2016) (PubMed).

Perrone, Butovskaya, Lago, Garzino-Demo, Pannecouque, Palù, Richter: "The G-quadruplex-forming aptamer AS1411 potently inhibits HIV-1 attachment to the host cell." in: International journal of antimicrobial agents, Vol. 47, Issue 4, pp. 311-6, (2016) (PubMed).

Tosoni, Frasson, Scalabrin, Perrone, Butovskaya, Nadai, Palù, Fabris, Richter: "Nucleolin stabilizes G-quadruplex structures folded by the LTR promoter and silences HIV-1 viral transcription." in: Nucleic acids research, Vol. 43, Issue 18, pp. 8884-97, (2015) (PubMed).

Target Details

Target: Nucleolin (NCL)

Alternative Name: Nucleolin

Background: Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.

Molecular Weight: 76.4 kDa

NCBI Accession: NP_005372

Pathways: Ribonucleoprotein Complex Subunit Organization