OTC Protein (DYKDDDDK Tag)

Catalog No. ABIN2727963

This Recombinant OTC protein is produced in Insect cells (Sf9).

Quick Overview for OTC Protein (DYKDDDDK Tag) (ABIN2727963)

Target: OTC (Ornithine Carbamoyltransferase (OTC))

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This OTC protein is labelled with DYKDDDDK Tag.

Characteristics:

• Recombinant human Ornithine carbamoyltransferase (C-term DDK tag) protein expressed in Sf9 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 50 mM Tris-HCl, pH 8.0, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: OTC (Ornithine Carbamoyltransferase (OTC))

Alternative Name: Ornithine Carbamoyltransferase

Background: This nuclear gene encodes a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia. Since the gene for this enzyme maps close to that for Duchenne muscular dystrophy, it may play a role in that disease also.

Molecular Weight: 36.1 kDa

NCBI Accession: NP_000522