PAK6 Protein (Transcript Variant 1)
Quick Overview for PAK6 Protein (Transcript Variant 1) (ABIN2728179)
Target
See all PAK6 ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
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Characteristics
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- Recombinant human PAK6 (transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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Endotoxin Level
- <0.1 ng/μg of protein (<1EU/μg).
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Biological Activity Comment
- Specific activity was determined as 2,086 pmoles/min/μg, according to the Zlyte assay protocol
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P21-Activated Kinase 6 (PAK6) (AA 1-681) protein (His tag)
custom-made PAK6 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
P21-Activated Kinase 6 (PAK6) (AA 1-682) protein (His tag)custom-made PAK6 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
P21-Activated Kinase 6 (PAK6) (AA 1-681) protein (GST tag)PAK6 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
Restrictions
- For Research Use only
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Concentration
- 1 mg/mL
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Buffer
- 25 mM Tris-HCl pH 8.0, 150 mM NaCl, 10 % glycerol, 5 mM DTT.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- PAK6 (P21-Activated Kinase 6 (PAK6))
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Alternative Name
- Pak6
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Background
- This gene encodes a member of a family of p21-stimulated serine/threonine protein kinases, which contain an amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. These kinases function in a number of cellular processes, including cytoskeleton rearrangement, apoptosis, and the mitogen-activated protein (MAP) kinase signaling pathway. The protein encoded by this gene interacts with androgen receptor (AR) and translocates to the nucleus, where it is involved in transcriptional regulation. Changes in expression of this gene have been linked to prostate cancer. Alternative splicing results in multiple transcript variants.
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Molecular Weight
- 34.2 kDa
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NCBI Accession
- NP_064553
Target
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