Paired Box 3 Protein (PAX3) (His tag)
Quick Overview for Paired Box 3 Protein (PAX3) (His tag) (ABIN2728312)
Target
See all Paired Box 3 (PAX3) ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This Paired Box 3 protein is labelled with His tag.
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Characteristics
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- Recombinant human PAX3 (N-term HIS tag, transcript variant PAX3D) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
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Paired Box 3 (PAX3) (AA 1-479) protein (His tag)
custom-made PAX3 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Paired Box 3 (PAX3) (AA 1-479) protein (His tag)custom-made PAX3 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Paired Box 3 (PAX3) (AA 1-484) protein (GST tag)PAX3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Paired Box 3 (PAX3) protein (Myc-DYKDDDDK Tag)PAX3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the N-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Paired Box 3 (PAX3)
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Alternative Name
- Pax3
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Background
- This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(213)(q35q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini.
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Molecular Weight
- 53.3 kDa
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NCBI Accession
- NP_852123
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Pathways
- Sensory Perception of Sound, Tube Formation
Target
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