PDE5A Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2728518

This Recombinant PDE5A protein is produced in HEK-293 Cells.

Quick Overview for PDE5A Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag) (ABIN2728518)

Target: PDE5A (phosphodiesterase 5A, cGMP-Specific (PDE5A))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 2

Purification tag / Conjugate: This PDE5A protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human PDE5A (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: PDE5A (phosphodiesterase 5A, cGMP-Specific (PDE5A))

Alternative Name: Pde5a

Background: This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5&apos-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms.

Molecular Weight: 94.6 kDa

NCBI Accession: NP_236914

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling