PEMT Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)
Quick Overview for PEMT Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag) (ABIN2728652)
Target
See all PEMT ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 2
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Purification tag / Conjugate
- This PEMT protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human PEMT (transcript variant 2) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Phosphatidylethanolamine N-Methyltransferase (PEMT) (AA 1-236) protein (GST tag)
PEMT Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Phosphatidylethanolamine N-Methyltransferase (PEMT) (AA 1-167) (Active) protein (His-Fc tag)PEMT Origin: Human Host: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. ELISA, WB, AcA, Imm, SDS Active
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- PEMT (Phosphatidylethanolamine N-Methyltransferase (PEMT))
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Alternative Name
- Pemt
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Background
- Phosphatidylcholine (PC) is the most abundant mammalian phospholipid. This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. Another distinct synthetic pathway in nucleated cells converts intracellular choline to phosphatidylcholine by a three-step process. The protein isoforms encoded by this gene localize to the endoplasmic reticulum and mitochondria-associated membranes. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms.
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Molecular Weight
- 22 kDa
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NCBI Accession
- NP_009100
Target
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