PHGDH Protein (DYKDDDDK Tag)

Catalog No. ABIN2728837

This Recombinant PHGDH protein is expressed in Insect cells (Sf9).

Quick Overview for PHGDH Protein (DYKDDDDK Tag) (ABIN2728837)

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This PHGDH protein is labelled with DYKDDDDK Tag.

Characteristics:

• Recombinant human PHGDH (full length, C-term DDK tag) protein expressed in Sf9 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 50 mM Tris-HCl, pH 8.0, 100 mM glycine, 10 % glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Alternative Name: Phgdh

Background: This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known.

Molecular Weight: 56.7 kDa

NCBI Accession: NP_006614

Pathways: Metabolism of Steroid Hormones and Vitamin D, Warburg Effect