PHGDH Protein (DYKDDDDK Tag)
Quick Overview for PHGDH Protein (DYKDDDDK Tag) (ABIN2728837)
Target
See all PHGDH ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This PHGDH protein is labelled with DYKDDDDK Tag.
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Characteristics
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- Recombinant human PHGDH (full length, C-term DDK tag) protein expressed in Sf9 cells.
- Produced with end-sequenced ORF clone
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phosphoglycerate Dehydrogenase (PHGDH) (AA 1-533) protein (GST tag)
PHGDH Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
phosphoglycerate Dehydrogenase (PHGDH) (AA 1-533) protein (His-IF2DI Tag)PHGDH Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
phosphoglycerate Dehydrogenase (PHGDH) (AA 2-251), (partial) protein (GST tag)PHGDH Origin: Human Host: Escherichia coli (E. coli) Recombinant 95 % ELISA
phosphoglycerate Dehydrogenase (PHGDH) protein (Myc-DYKDDDDK Tag)PHGDH Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 50 mM Tris-HCl, pH 8.0, 100 mM glycine, 10 % glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- PHGDH (phosphoglycerate Dehydrogenase (PHGDH))
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Alternative Name
- Phgdh
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Background
- This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known.
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Molecular Weight
- 56.7 kDa
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NCBI Accession
- NP_006614
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Pathways
- Metabolism of Steroid Hormones and Vitamin D, Warburg Effect
Target
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