Phospholipase B Protein (PLB) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2728861

This Recombinant Phospholipase B protein is expressed in HEK-293 Cells.

Quick Overview for Phospholipase B Protein (PLB) (Myc-DYKDDDDK Tag) (ABIN2728861)

Target: Phospholipase B (PLB)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This Phospholipase B protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human Phospholipase B1 (PLB1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: Phospholipase B (PLB)

Alternative Name: Phospholipase b1 (Plb1)

Background: This gene encodes a membrane-associated phospholipase that displays lysophospholipase and phospholipase A2 activities through removal of sn-1 and sn-2 fatty acids of glycerophospholipids. In addition, it displays lipase and retinyl ester hydrolase activities. The encoded protein is highly conserved and is composed of a large, glycosylated extracellular domain composed of four tandem homologous domains, followed by a hydrophobic segment that anchors the enzyme to the membrane and a short C-terminal cytoplasmic tail. This gene has been identified as a candidate rheumatoid arthritis risk gene.

Molecular Weight: 162.9 kDa

NCBI Accession: NP_694566