PIM1 Protein (Myc-DYKDDDDK Tag)
Quick Overview for PIM1 Protein (Myc-DYKDDDDK Tag) (ABIN2728984)
Target
See all PIM1 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This PIM1 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human PIM1 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Pim-1 Oncogene (PIM1) (AA 38-290) protein (His tag)
PIM1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Pim-1 Oncogene (PIM1) (AA 1-313) protein (GST tag)PIM1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- PIM1 (Pim-1 Oncogene (PIM1))
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Alternative Name
- Pim1
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Background
- The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805, 1825810).[provided by RefSeq, Aug 2011].
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Molecular Weight
- 35.5 kDa
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NCBI Accession
- NP_002639
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Pathways
- Glycosaminoglycan Metabolic Process
Target
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