PLOD1 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2729187

This Recombinant PLOD1 protein is produced in HEK-293 Cells.

Quick Overview for PLOD1 Protein (Myc-DYKDDDDK Tag) (ABIN2729187)

Target: PLOD1 (Procollagen-Lysine,2-Oxoglutarate 5-Dioxygenase 1 (PLOD1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This PLOD1 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human PLOD1 / PLOD protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: PLOD1 (Procollagen-Lysine,2-Oxoglutarate 5-Dioxygenase 1 (PLOD1))

Alternative Name: Plod1,plod

Background: Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 81.5 kDa

NCBI Accession: NP_000293