POLR2J2 Protein (Myc-DYKDDDDK Tag)
Quick Overview for POLR2J2 Protein (Myc-DYKDDDDK Tag) (ABIN2729325)
Target
See all POLR2J2 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This POLR2J2 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human POLR2J2 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Polymerase (RNA) II (DNA Directed) Polypeptide J2 (POLR2J2) (AA 1-115) protein (His tag)
custom-made POLR2J2 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Polymerase (RNA) II (DNA Directed) Polypeptide J2 (POLR2J2) (AA 1-115) protein (GST tag)POLR2J2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Polymerase (RNA) II (DNA Directed) Polypeptide J2 (POLR2J2) (AA 1-116) protein (GST tag)POLR2J2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Polymerase (RNA) II (DNA Directed) Polypeptide J2 (POLR2J2) proteinPOLR2J2 Origin: Human Host: Escherichia coli (E. coli) Recombinant SDS
Polymerase (RNA) II (DNA Directed) Polypeptide J2 (POLR2J2) (AA 1-115) protein (His-IF2DI Tag)POLR2J2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Polymerase (RNA) II (DNA Directed) Polypeptide J2 (POLR2J2) (AA 1-115) protein (His tag)POLR2J2 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % SDS
Polymerase (RNA) II (DNA Directed) Polypeptide J2 (POLR2J2) protein (His tag)POLR2J2 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- POLR2J2 (Polymerase (RNA) II (DNA Directed) Polypeptide J2 (POLR2J2))
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Alternative Name
- Polr2j2
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Background
- This gene is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of this family, DNA directed RNA polymerase II polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple, alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD). Consequently, it is not known if this locus expresses a protein or proteins in vivo.
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Molecular Weight
- 12.9 kDa
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NCBI Accession
- NP_116581
Target
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