Quick Overview for POLR2K Protein (Myc-DYKDDDDK Tag) (ABIN2729327)
Target
See all POLR2K ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This POLR2K protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human POLR2K protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Polymerase (RNA) II (DNA Directed) Polypeptide K, 7.0kDa (POLR2K) (AA 1-58) protein (Fc Tag)
custom-made POLR2K Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Polymerase (RNA) II (DNA Directed) Polypeptide K, 7.0kDa (POLR2K) (AA 1-58) protein (Fc Tag)custom-made POLR2K Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Polymerase (RNA) II (DNA Directed) Polypeptide K, 7.0kDa (POLR2K) (AA 1-58) protein (GST tag)POLR2K Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Polymerase (RNA) II (DNA Directed) Polypeptide K, 7.0kDa (POLR2K) (AA 1-58) protein (His-IF2DI Tag)POLR2K Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Polymerase (RNA) II (DNA Directed) Polypeptide K, 7.0kDa (POLR2K) (AA 1-58) protein (His tag)POLR2K Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- POLR2K (Polymerase (RNA) II (DNA Directed) Polypeptide K, 7.0kDa (POLR2K))
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Alternative Name
- Polr2k
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Background
- This gene encodes one of the smallest subunits of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases.
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Molecular Weight
- 6.8 kDa
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NCBI Accession
- NP_005025
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Pathways
- Regulatory RNA Pathways
Target
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