Quick Overview for PPM1F Protein (Myc-DYKDDDDK Tag) (ABIN2729807)
Target
See all PPM1F ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This PPM1F protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human Protein phosphatase 1F / PPM1F protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F) (AA 1-452) protein (His tag)
custom-made PPM1F Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F) (AA 1-454) protein (GST tag)PPM1F Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F) (AA 1-454) protein (His-IF2DI Tag)PPM1F Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F) (AA 1-454) protein (His tag)PPM1F Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- PPM1F (Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F))
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Alternative Name
- Protein Phosphatase 1f,ppm1f
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Background
- The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined.
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Molecular Weight
- 49.7 kDa
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NCBI Accession
- NP_055449
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Pathways
- Positive Regulation of Endopeptidase Activity
Target
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