PSMF1 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2730015

This Recombinant PSMF1 protein is produced in HEK-293 Cells.

Quick Overview for PSMF1 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag) (ABIN2730015)

Target: PSMF1 (Proteasome (Prosome, Macropain) Inhibitor Subunit 1 (PI31) (PSMF1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 2

Purification tag / Conjugate: This PSMF1 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human PSMF1 / PI31 (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: PSMF1 (Proteasome (Prosome, Macropain) Inhibitor Subunit 1 (PI31) (PSMF1))

Alternative Name: Psmf1,pi31

Background: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene.

Molecular Weight: 29.6 kDa

NCBI Accession: NP_848693

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA