PUF60 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
(1 reference)Quick Overview for PUF60 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2730160)
Target
See all PUF60 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Purification tag / Conjugate
- This PUF60 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human PUF60 / SIAHBP1 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Poly-U Binding Splicing Factor 60KDa (PUF60) (AA 1-559) protein (His tag)
custom-made PUF60 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Poly-U Binding Splicing Factor 60KDa (PUF60) (AA 1-564) protein (His tag)custom-made PUF60 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Poly-U Binding Splicing Factor 60KDa (PUF60) (AA 1-516) protein (GST tag)PUF60 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Poly-U Binding Splicing Factor 60KDa (PUF60) (AA 1-499) protein (GST tag)PUF60 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Poly-U Binding Splicing Factor 60KDa (PUF60) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)PUF60 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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: "PUF60: a prominent new target of the autoimmune response in dermatomyositis and Sjögren's syndrome." in: Annals of the rheumatic diseases, Vol. 75, Issue 6, pp. 1145-51, (2016) (PubMed).
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: "PUF60: a prominent new target of the autoimmune response in dermatomyositis and Sjögren's syndrome." in: Annals of the rheumatic diseases, Vol. 75, Issue 6, pp. 1145-51, (2016) (PubMed).
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- PUF60 (Poly-U Binding Splicing Factor 60KDa (PUF60))
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Alternative Name
- Puf60,siahbp1
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Background
- This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
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Molecular Weight
- 59.7 kDa
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NCBI Accession
- NP_510965
Target
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