PUMA Protein (Transcript Variant 4) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2730161

Product Details

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Protein Type: Recombinant

Protein Characteristics: Transcript Variant 4

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This PUMA protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human PUMA (transcript variant 4) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Alternative Name: Puma (BBC3 Products)

Background: This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants.

Molecular Weight: 20.4 kDa

NCBI Accession: NP_055232

Pathways: p53 Signaling, Positive Regulation of Endopeptidase Activity