PUMA Protein (AA 141-261) (His-IF2DI Tag)
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- Target See all PUMA (BBC3) Proteins
- PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 141-261
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PUMA protein is labelled with His-IF2DI Tag.
- Application
- Western Blotting (WB), ELISA
- Purity
- Greater than 90 % by SDS-PAGE gel analyses
- Sterility
- 0.2 μm filtered
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BCL2 Binding Component 3 (BBC3) (AA 1-193) protein (Strep Tag)
Crystallography grade BBC3 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
BCL2 Binding Component 3 (BBC3) (AA 1-261) protein (Strep Tag)Crystallography grade BBC3 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
BCL2 Binding Component 3 (BBC3) (AA 1-193) protein (Strep Tag)Crystallography grade BBC3 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
BCL2 Binding Component 3 (BBC3) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)BBC3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
BCL2 Binding Component 3 (BBC3) (AA 76-170) protein (His tag,Strep II tag)BBC3 Origin: Human Host: Escherichia coli (E. coli) Recombinant ~95 % WB, ELISA, MS
BCL2 Binding Component 3 (BBC3) (Transcript Variant 4) protein (Myc-DYKDDDDK Tag)BBC3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/mL in sterile distilled water by gentle pipetting 2-3 times, don't vortex
- Buffer
- Lyophilized from a 0.2 μm filtered solution in PBS with 5 % trehalose, pH 7.4
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C for 12 months as lyophilized, 2-8°C for 1 month under sterile conditions after reconstitution
- Expiry Date
- 12 months
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- Target
- PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
- Alternative Name
- PUMA (BBC3 Products)
- Synonyms
- BBC3 Protein, Puma Protein, JFY-1 Protein, JFY1 Protein, PUMA Protein, PUMA/JFY1 Protein, BCL2 binding component 3 Protein, Bcl-2 binding component 3 Protein, BBC3 Protein, Bbc3 Protein, bbc3 Protein
- Background
- BBC3, JFY 1, JFY1, PUMA
- Molecular Weight
- 32.5 kDa
- UniProt
- Q96PG8
- Pathways
- p53 Signaling, Positive Regulation of Endopeptidase Activity
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