RASGRP1 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2730515

Recombinant RASGRP1 protein expressed in HEK-293 Cells.

Quick Overview for RASGRP1 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2730515)

Target: RASGRP1 (RAS Guanyl Releasing Protein 1 (Calcium and DAG-Regulated) (RASGRP1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 1

Purification tag / Conjugate: This RASGRP1 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human RAS guanyl releasing protein 1 (calcium and DAG-regulated) (RASGRP1), transcript variant 1 (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: RASGRP1 (RAS Guanyl Releasing Protein 1 (Calcium and DAG-Regulated) (RASGRP1))

Background: This gene is a member of a family of genes characterized by the presence of a Ras superfamily guanine nucleotide exchange factor (GEF) domain. It functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. It activates the Erk/MAP kinase cascade and regulates T-cells and B-cells development, homeostasis and differentiation. Alternatively spliced transcript variants encoding different isoforms have been identified. Altered expression of the different isoforms of this protein may be a cause of susceptibility to systemic lupus erythematosus (SLE).

Molecular Weight: 90.2 kDa

NCBI Accession: NP_005730

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway